Date: July 2022
Title: Research Scientist
Department: Pulmonary & Critical Care
Location: BRB Building, 3rd Floor
Supervisor Name and Title: Kenneth E. Remy, MD,MHSc, MSCi, FCCM; Associate Professor
The Remy Host Immune Response Laboratory elucidates and identifies mechanistic and causal pathways in diseases that cause host immune dysregulation including SARS-CoV-2 infection and sepsis (adult and pediatric. Understanding these mechanisms will enhance current understanding of host innate and adaptive immune responses in COVID disease and sepsis, and enable the application and identification of potential targets for disease intervention including the use of immunoadjuvants. My current work explores two areas: heme/iron-based trafficking the focus of my NIH funded NIGMS K08 and signaling in immune dysregulation in context of diseases of intravascular hemolysis (COVID, sepsis, malaria, sickle cell disease, thalassemia) and after red blood cell transfusion; and real time immunophenotyping of pro and hypoinflammatory states to identify timing for immunoadjuvant therapies. Additionally, the laboratory investigates novel immunoadjuvant therapies for sepsis in adults and children including the use of IL-7 and PD-L1. The use of real-time immunophenotyping assay platforms such as our use of whole blood ELISpot assay are currently in development to evaluate response of therapeutics from an in vitro translational model from a patient and back to the patient.
The Research Scientist will oversee research projects within the lab, providing scientific oversight, guidance to lab members, and lab management in the absence of the principal investigator. The Research Scientist will design and plan projects, oversee collaborations, oversee/supervise staff and students, manage animal models, and fully execute research projects in the field of hemolysis, immune endotyping, deep immune profiling in both human and animal models. The Research Scientist will utilize PCR, Flow Cytometry, ELISA, ssRNASeq and Bulk RNA sequencing and additional biochemical medthods. The Research Scientist will keep the PI informed of progress of research projects and provide recommendations on experimental design and project plans. The Research Scientist will independently prepare manuscripts. It is expected that the Research Scientist will apply for grant funding as a Principal Investigator in this area of interest and maintain independent research though submission of subsequent grant applications an needed. The Research Scientist will also participate collaboratively with other Principal Investigators within and external to CWRU.
ESSENTIAL FUNCTIONSProvide scientific oversight of all research projects, including data collection and maintenance. Establish and manage the day-to-day priorities of the research staff.Independently write 3 grants per year with the PI including one grant with the RS as PI. Independently write and apply for grants as Principal Investigator. Submit subsequent grant applications as needed to maintain independent research and funding.Provide independent writing of manuscripts and assistance with editing current manuscripts for PI and laboratory personnel.With the principal investigator, establish goals and objectives for the efficient and timely completion of projects. Meet regularly with the principal investigator to review and set priorities, staff/student-related issues, and future research staff and planning.Independently and with the principal investigator, design, plan, and execute research projects within the aims of new and ongoing projects. Perform analysis of flow cytometry, Western blotting, ELISA, DNA and RNA isolation, PCR, in vivo studies using animals. Maintain accurate records of data and provide findings and recommendations to the PI based on analysis.Develop new techniques and protocols to advance research projects within the lab. Train lab members on new techniques. Coordinate experiments performed by other lab members, ensuring adequate resources, assisting with experimental design and analysis. Oversee progress of the research projects and provide regular progress reports to the PI; and provide input and recommendation to principal investigator regarding significant developments in research projects. Ensure the PI is informed about the results of lab members, and ensure the PI’s experimental strategy is explored by lab members and through independent experiments by the Research Scientist.Mentor and train lab staff and graduate students; oversee research work and ensure pro jects are being completed according to the research plan. This will involve teaching other members of the lab techniques and scientific discovery methods, including students and post-docs. Assist and teach both established and new research techniques to other lab members to facilitate their experiments. Coordinate trainings for lab members with the EHS and others, as needed.Present data and findings at laboratory meetings and scientific meetings, seminars, etc. Prepare manuscripts, and contribute preliminary data for grant proposals. Independently author research projects. Assemble data and make publication-quality figures from analyzed data. With the highest level of integrity and responsible conduct of research, participate in writing manuscripts as a first author and co-author for discoveries from research performed in the lab, together with the PI and other lab members.Assess, update, and develop new standard operating procedures (SOPs) for the lab.Supervise research assistants and technicians. Supervise the research and technical work performed by other lab members, ensuring proper lab procedures are followed, and their safety, and ensuring adequate resources are provided for lab work.Operate laboratory equipment such as flow cytometer. ssist other lab members with general lab maintenance and help oversee and maintain inventory of scientific reagents.
NONESSENTIAL FUNCTIONSPerform other duties as assigned.
Department: Frequent contact with PI and lab members, may work with other faculty for collaboration of research projects, contact with administrative staff as needed
University: Regular/Occasional contact with other labs for collaboration, Regular/Occasional contact with the Animal Resource Center, Contact with other departments as needed
External: Supply vendors, other institutions, funding agencies, etc. as needed
Students: Undergraduate and graduate student employees working in the lab
Supervise laboratory staff including research assistants and technicians. May supervise Research Associates within the lab. Oversee all lab members including postdoctoral fellows, graduate, and undergraduate students.
Experience: Minimum of 5 years of related postdoctoral experience in preferred field(s) of study preferred. Less experience and demonstrated expertise in a particular field of study will be considered.
Education/Licensing: PhD, MD, or other terminal degree (indicate accepted terminal degrees) in (specified) field of study.
REQUIRED KNOWLEDGE, SKILLS and ABILITIES Demonstrated ability to work independently and communicate with laboratory staff and investigators.Ability to maintain complete and organized records/reports. Experience with hematology and immunology based assays including use of flow cytometry and ELISA.Excellent analytical skills; ability to formulate findings and recommendations from the analysis.Excellent oral and written communication skills and interpersonal skills; must demonstrate the ability to effectively and professionally communicate and work with various individuals within and external to the University. Excellent technical writing skills required.Strong organization skills; ability to multi-task, prioritize, and meet deadlines. Must demonstrate attention to detail and accuracy, time management skills, and follow-through. Must be able to work under pressure and conform to shifting priorities, demands, and timeline. Effective leadership skills; ability to lead, train, work with, and elicit cooperation from team members and staff. Ability to work effectively independently and collaboratively within a team. Must be highly motivated, responsible, dependable, and a self-starter.Effective problem-solving skills; must demonstrate innovation and creativity, sound judgment, and good decision-making. Good computer skills, including database management. Ability to learn new programs as needed.Animal research only: Must demonstrate compliance with CWRU animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.Animal research only: Must demonstrate compassion for animals within CWRU facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.Animal Research only: Must be able and willing to maintain a high standard of personal cleanliness and utilize protective gear to protect the health the animals.
This position will be a Monday-Friday position with rare need for a weekend assay to be completed. This will be discussed with the PI. The position entails normal business hour working with rare but occasional travel.
Animal research only: Case's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.
In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity. Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.
Interested applicants should submit their CV and cover letter to Associate Professor Kenneth Remy at KXR53@case.edu.