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Senior Stem Cell Lab Researcher - 114338
University of California, San Diego in La Jolla, California
Date Posted 05/26/2022
Research Assistant-Biology
Employment Type Fulltime
Application Deadline Open until filled

Senior Stem Cell Lab Researcher - 114338

UCSD Layoff from Career Appointment: Apply by 02/03/2022 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 02/15/2022. Eligible Special Selection clients should contact their Disability Counselor for assistance.

This position will remain open until a successful candidate has been identified.

The Department of Medicine is responsible for fulfilling the teaching, research, and clinical missions of the University of California, San Diego and employs 500+ full-time salaried faculty members and 150+ academic appointees. Staff personnel include 800+ staff in 9 bargaining units, students, and volunteers. There are over 250 clinical faculties practicing at multiple sites and hospitals. Professional fee billings for the department exceed $50 million with collections of over $19 million per year. The total annual budget is approximately $200 million, which includes contract and grant revenues, clinical practice and medical center funds, and other state and private endowment funds.

Independently conduct research projects based on reviews of scientific literature and recent advances in related fields while contributing original/novel ideas of major methodological significance to projects, designing key experiments and developing new techniques. Specifically, manage the milestones of long-term projects involving cardiovascular development, differentiation and diseases utilizing mammalian and human pluripotent stem cell (hPSC) systems. Plan, execute and follow-up on high level regular and ad hoc meetings of investigators and management. Author or co-author scientific journal manuscripts and book chapters. Report directly to funding agencies and journal editors. Prepare and implement research study budgets based on analysis of data. Direct, operate and maintain a cardiovascular stem cell core, including overseeing human induced pluripotent stem cell colonies, maintaining a database of all fibroblast and hPSC strains, ordering reagents for stem cell work as well as supervising lab personnel using this stem cell core.

Manage a team in directing the cardiovascular stem cell core and provide reports after analyses has been performed. Perform other responsibilities pertaining to job, including strategic and operational support, updating quarterly/annual progress reports, conducting evaluation meetings, monitoring progress via written reports and teleconferences, and establishing oversight advisory committees. Perform advanced novel cell biology, molecular biology and protein analysis. Conduct sample preparation from next-generation sequencing platforms. Create, maintain, transfect, select and analyze transgenic cardiovascular hPSC lines using different techniques including CRISPR/Cas9-based methods. Stay up to date on current and emerging techniques for cell line generation. Perform directed cardiovascular differentiations using different protocols. Direct and advise the work of technicians, students, and post-doctoral fellows to enable them to successfully participate in laboratory projects. Independently write grant proposals to obtain further funding for experimental projects. Perform other duties as needed.

  • Advanced theoretical knowledge of stem cells, biochemistry, cellular and molecular biology, including experience in establishing, maintaining, transfecting, and selecting clones from primary patients samples or hPSCs with expertise in sterile technique.

  • Proven skill in experimental design and adaptation of methods to achieve results. Ability to integrate data from multiple sources to synthesize conclusions. Ability to read and abstract scientific articles and apply to the design of experiments.

  • Strong experience in contributing and implementing innovative ideas and techniques on complex and multi-disciplinary research projects.

  • Advanced experience in designing, coordinating, executing, and analyzing data.

  • Proven experience and knowledge in developing hypotheses, design experiments, conduct experiments, analyze data, write manuscripts, and publish results in peer-reviewed journals, including all interactions with Reviewers and Editors.

  • Experience in managing multi-center projects and working under milestones-driven projects, including knowledge in writing reports and experience in communicating with the funding agency.

  • Excellent written, verbal and interpersonal communication skills including tack, diplomacy, and flexibility in order to work and interact effectively with staff, researchers, and management in person and on the phone.

  • Demonstrated experience in DNA and RNA extraction and use of extracted products in various PCR methods.

  • Superior experience in generating SOP and documentation required to provide guideline to team members. Skills to organize meetings and conferences.

  • Experience with UCSD regulations, policies, and procedures.
  • Experience with generating cell lines using CRISPR/Cas9.
  • Experience with stem cell differentiation protocols. Experience with human pluripotent stem cell culture.
  • Experience in procuring funding by designing and executing experiments, analyzing data and deriving conclusions, developing experimental plans based on results from those preliminary studies, and writing all components of grant applications.
  • Employment is subject to a criminal background.
  • Must be willing to work extended hours.
  • Must be willing to work with potentially hazardous materials.

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

UC San Diego Health Sciences is comprised of our School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, The Herbert Wertheim School of Public Health and Human Longevity Science, and our Student Health and Well-Being Department. We have long been at the forefront of translational - or "bench-to-bedside" - research, transforming patient care through discovery and innovation leading to new drugs and technologies. Translational research is carried out every day in the hundreds of clinical trials of promising new therapies offered through UC San Diego Health, and in the drive of our researchers and clinician-scientists who are committed to having a significant impact on patient care. We invite you to join our team!

Applications/Resumes are accepted for current job openings only. For full consideration on any job, applications must be received prior to the initial closing date. If a job has an extended deadline, applications/resumes will be considered during the extension period; however, a job may be filled before the extended date is reached.

UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information.

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