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Field of Research:
Immunology & Infectious Disease
The University of Washington Division of Allergy and Infectious Diseases is seeking a full-time Research Scientist/Engineer 2 at the UW Research Campus in South Lake Union. The lab is investigating immunologic mechanisms of disease pathogenesis with an emphasis on genetic, cellular, and molecular studies of the innate immune response. These studies are directed towards understanding why individuals have different susceptibility to infections and whether these insights can lead to novel treatment and vaccine strategies. Our primary target pathogens are Mycobacterium tuberculosis, Legionella, and Mycobacterium leprae.
Research Sponsors and Stakeholders:
NIH, Bill and Melinda Gates Foundation
55% Cell culture and molecular biology work
• Induced pluripotent stem cell work (iPSC): Derive and reprogram iPSCs and differentiate into myeloid-like cells
• Conduct ELISA assays to measure cytokine production
• Perform flow cytometry to identify specific cell markers and cytokine-producing cells.
• Molecular biology: Examples of experiments include basic cloning techniques, DNA and RNA purification, restriction enzyme digestions, PCR, Western blots, cell viability assays
• Cell culture work: Prepare, culture, transfect and stimulate cells in vitro. Cryopreservation and thawing of cells. Purification of white blood cell fractions from human blood.
• Gene editing: use siRNA and CRISPR to knock down gene function in myeloid cells
• Bacteria culture work: growing, maintaining, and using bacteria to infect cells
30% Data collection/analysis and collaboration
• Record keeping, data collection and analysis.
• Collaborative interaction: Extensive interaction with others in the lab. Work closely with postdoctoral fellows on their research projects
• Consenting participants and helping manage an ongoing study
• Training undergraduates or new lab members in tissue culture and basic molecular biology.
15% Laboratory management
• Order reagents and keep track of laboratory supplies
• General lab maintenance: maintaining incubators, refilling water baths, disposing of biohazardous waste, etc.
Bachelor's Degree biochemistry, molecular biology, cellular biology, immunology, microbiology, or related field. At least 2 years of experience to include:
• Highly organized and have knowledge of laboratory working environments
• Experience in cell culture and/or immunology BS level of understanding of knowledge of microbiology and immunology
• Able to identify technical problems in experiments and find solutions to basic problems
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