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RESEARCH SCIENTIST/ENGINEER 2
University of Washington in Seattle, Washington
 
 
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Date Posted 03/20/2022
Category
Admin-Laboratory and Research
Employment Type
Application Deadline Open until filled
 
 
 
 
 

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. 

Field of Research:

Immunology & Infectious Disease

Research Purpose:

The University of Washington Division of Allergy and Infectious Diseases is seeking a full-time Research Scientist/Engineer 2 at the UW Research Campus in South Lake Union.  The lab is investigating immunologic mechanisms of disease pathogenesis with an emphasis on genetic, cellular, and molecular studies of the innate immune response.  These studies are directed towards understanding why individuals have different susceptibility to infections and whether these insights can lead to novel treatment and vaccine strategies.  Our primary target pathogens are Mycobacterium tuberculosis, Legionella, and Mycobacterium leprae.

Research Sponsors and Stakeholders:

NIH, Bill and Melinda Gates Foundation

RESPONSIBILITIES:

55% Cell culture and molecular biology work

• Induced pluripotent stem cell work (iPSC):  Derive and reprogram iPSCs and differentiate into myeloid-like cells
• Conduct ELISA assays to measure cytokine production
• Perform flow cytometry to identify specific cell markers and cytokine-producing cells.
• Molecular biology: Examples of experiments include basic cloning techniques, DNA and RNA purification, restriction enzyme digestions, PCR, Western blots, cell viability assays
• Cell culture work: Prepare, culture, transfect and stimulate cells in vitro. Cryopreservation and thawing of cells. Purification of white blood cell fractions from human blood.
• Gene editing:  use siRNA and CRISPR to knock down gene function in myeloid cells
• Bacteria culture work: growing, maintaining, and using bacteria to infect cells

30% Data collection/analysis and collaboration
• Record keeping, data collection and analysis.
• Collaborative interaction: Extensive interaction with others in the lab. Work closely with postdoctoral fellows on their research projects
• Consenting participants and helping manage an ongoing study
• Training undergraduates or new lab members in tissue culture and basic molecular biology.

15% Laboratory management

• Order reagents and keep track of laboratory supplies
• General lab maintenance: maintaining incubators, refilling water baths, disposing of biohazardous waste, etc.

MINIMUM REQUIREMENTS:

Bachelor's Degree biochemistry, molecular biology, cellular biology, immunology, microbiology, or related field. At least 2 years of experience to include:
• Highly organized and have knowledge of laboratory working environments
• Experience in cell culture and/or immunology BS level of understanding of knowledge of microbiology and immunology
• Able to identify technical problems in experiments and find solutions to basic problems

Application Process:

The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process.  These assessments may include Workforce Authorization, Cover Letter and/or others.  Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.


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