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RESEARCH SCIENTIST 2
University of Washington in Seattle, Washington
 
 
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Date Posted 03/12/2022
Category
Admin-Laboratory and Research
Employment Type
Application Deadline Open until filled
 
 
 
 
 

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.

The Division of Medical Genetics has an exciting job opportunity for a Research Scientist 2 with an interest in developmental and degenerative neurogenetics. We study both single gene and complex disorders. The work involves discovery of disease-causing genes/variants, and functional analyses in various biological models, including cell lines, transgenic mice, and induced pluripotent stem cells (iPCS).

The applicant should be a motivated, highly organized individual interested in learning/applying novel skills and techniques in this fast-moving field. The Research Scientist 2 is expected to work independently and collaboratively with a highly interactive group including the PI, faculty colleagues, research personnel and postdoctoral fellows.

RESPONSIBILITIES
• Manage the mouse colony, including ordering and establishing mouse lines, process mouse tails for DNA, PCR-based genotyping, process mouse tissues for other analyses, and maintain the mouse-model related databases - 40%
• Process human blood samples, maintain the human sample log and database, separate blood cell fractions, extract DNA and RNA and establish and maintain EBV transformed lymphocyte lines - 15%
• Cell culture, growth analyses, flow cytometry, and related assays - 10%
• Perform mouse neurobehavioral tests - 10%
• Administrative activities: ordering supplies, lab housekeeping, renewing permits - 10%
• Present ongoing work and findings at lab meetings - 5%
• Participate in preparation of manuscripts for publication - 5%
• Troubleshoot experimental problems - 5%

MINIMUM REQUIREMENTS
• Bachelor’s degree in biological science or related field. At least 2 years of relevant experience.

Additional requirements:
• Experience with basic lab procedures like DNA and RNA extraction from blood and tissues, PCR-based and next-generation sequence analysis and real-time PCR, DNA cloning and mutagenesis, cell culture, transfection and gene expression analysis, and western blot analyses.
• Expertise in genetics, molecular and cell biology, and mouse models.
• Experience working with mouse colonies.
• Experience with perfusion, stereotaxic surgery in mice, brain slice culture, CRISPR-Cas9 mutagenesis, iPSC, transcript profiling and proteomics, and confocal microscopy.
• Experience with light and fluorescence microscopy, mouse models, and tissue fixation, sectioning, and immunostaining.

Equivalent education and/or experience may substitute for minimum requirements.

DESIRED REQUIREMENTS
• Establishing and/or differentiation of human iPSCs.
• Experience with transcriptomics and proteomics.
• Advanced degree in a related field.

CONDITIONS OF EMPLOYMENT
• This position requires some flexibility of work schedule to accommodate experiments with fixed-time courses.

Application Process:
The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.


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