The University of Pittsburgh is seeking a Research Scientist to work in the laboratory of Dr. Warren Shlomchik at the UPMC Hillman Cancer Center. The candidate will study the mechanisms whereby interferon-gamma sensitizes leukemia cells to killing by alloreactive T cells. The project employs CRISPR-editing of novel mouse leukemia lines derived from compound oncogene knockin mice, single cell RNA sequencing, ATAC-seq, in vivo studies with novel T cell receptor transgenic mice, and CRISPR-Cas9-screens to identify genes that both render leukemia cells resistant to T cell killing.  Specific skills and knowledge that are essential for the position:1)      Knowledge of immunology, particularly cellular immunology is highly valued2)      Knowledge of malignant hematology is highly valued3)      Experience with mouse handling4)      Experience with CRISPR-Cas9 genome editing5)      Expertise in multi-parameter flow cytometry6)      Expertise in common molecular biology techniques including modern cloning, PCR, RT-PCR. 

PhD in a biological science with 5 years of postdoctoral training preferred. Specific skills and knowledge that are essential for the position:1)      Knowledge of immunology, particularly cellular immunology is highly valued2)      Knowledge of malignant hematology is highly valued3)      Experience with mouse handling4)      Experience with CRISPR-Cas9 genome editing5)      Expertise in multi-parameter flow cytometry6)      Expertise in common molecular biology techniques including modern cloning, PCR, RT-PCR.