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University of Washington in Seattle, Washington
Date Posted 06/10/2021
Admin-Laboratory and Research
Employment Type
Application Deadline Open until filled

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. 

The Department of Biochemistry at the University of Washington has contributed to many phases of research. Initially recognized for incisive studies of the structure and function of proteins, and the mechanisms of enzyme action, the department branched out into a multitude of other areas. These included: developmental biology of flies, fish, frogs, and mice; molecular mechanisms of aging in yeast and mammals; vertebrate visual and immune responses; chromosome dynamics, cell division, DNA replication and repair; energy transduction during photosynthesis; protein secretion and membrane fusion; translational control and regulated protein degradation; protein structure determination by crystallography and NMR spectroscopy; and protein structure prediction and enzyme design. Our diversity of interests guarantees a rich intellectual and educational environment.

The Institute for Protein Design (IPD) at the University of Washington in Seattle is creating a new world of synthetic proteins to address 21st-century challenges in medicine, energy, and technology. The IPD is a unique interdisciplinary environment where world-class researchers with expertise in computing and software, biochemistry, genome sciences, biological structure, pharmacology, immunology and other basic science disciplines, as well as clinical medicine, work together to both computationally design and experimentally characterize novel biomolecules. Current projects include the design of novel therapeutics for cancer, neurological diseases, and more, as well as new vaccines, nanomaterials, and enzymes.

The Institute for Protein Design has an outstanding opportunity for a Research Scientist/Engineer 1. 

1.  Main duties will include those described above, which will be carried out during normal working hours, periodically requiring overtime work to achieve key goals for the lab.
2. The Research Scientist 1 must be proficient with sterile laboratory maintenance and familiar with BSL2 requirements.
3. Will work closely with graduate students, postdoctoral fellows, and research staff in support of research involving protein expression, purification, and biophysical characterization
4. Reporting on all protein production steps will be compiled into our DesignTracker database for coordination between cores and researchers.
4. The Research Scientist 1 will be responsible for ensuring proper reagent stocks and for anticipating & avoiding supply shortcomings.
5. Maintaining cell lines will require excellent sterile technique, coordination with researchers, and careful planning.
4. Ensuring that DNA is purified, verified, and ready for transfections.
6. Evaluation of transfected cells, removal of BSL-2 biohazardous material, purification of target proteins from cell culture via column chromatography, and verification of presence via SDS-PAGE & ELISA.
7. Tracking cryo-preverved cell lines and on-boarding new lines from collaborators and suppliers.
8. Performing assays to detect and identify contamination of cultures.
9. Familiarity with office productivity software (Word, PowerPoint, Excel) to prepare reports on the work performed.

• Bachelor's Degree in Biochemistry or related field + 3 months or more years of experience.
• Organizational Scope and Impact of Position:
• Responsible for reliable production of mammalian proteins as part of core operations for a large protein research wet lab.
• Reliable production efforts and accurate reporting will enable core facility to efficiently produce target proteins for characterization and in vivo studies. Errors can impact multiple laboratories.
• Previous experience with protein expression and purification.

Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.

• 3 months-1 year of prior laboratory research experience.
• Willing to learn about protein engineering and design.
• Previous experience with biophysical methods for protein characterization using SDS-PAGE, native gel  electropheresis, ELISA or others.
• Previous experience working as part of a production team.
• Previous experience working in a BSL-2 environment.

As a UW employee, you will enjoy generous benefits and work/life programs.  For detailed information on Benefits for this position, click here.

Application Process:
The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process.  These assessments may include Workforce Authorization, Cover Letter and/or others.  Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

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