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Research Assistant IV
Harvard University in Cambridge, Massachusetts
Date Posted 05/14/2021
Admin-Laboratory and Research
Employment Type
Application Deadline Open until filled


Faculty of Arts and Sciences


Job Summary

Reporting to Professor Lee Rubin, Dept of Stem Cell and Regenerative Biology and the Harvard Stem Cell Institute, the Research Assistant will provide essential infrastructure to the skeletal muscle group. The candidate will collaborate with a team of researchers (scientists, postdoctoral fellows and research assistants) who will use small molecule and cell-based therapies to target skeletal muscle disorders. Experiments will involve in vitro cultures of mouse and human muscle, and standard molecular and cell biological techniques including immunohistochemistry, qPCR, and Western blotting. In addition, single cell RNAseq and ATACseq methodology will be employed. Finally, studies will be carried out to assess engraftment, integration and functional readouts using mouse models of skeletal muscle diseases.

Job-Specific Responsibilities

More specifically, Research Assistant will:

  • In line with the PI and Scientist group leader's research plan and project goals, the candidate will execute research experiments including completing research for multiple projects.
  • With expertise in extensive range of complex in vitro and in vivo assays, perform cell production, participate in the modifications and development of new protocols, procedures, techniques or applications of technology.
  • Conduct assays and make suggestions to optimally reflect relevant cellular behaviors. Screen chemical and other types of libraries to identify screenings with therapeutic relevance. 
  • Interpret screening hits into mechanistic insights and experimental hypotheses. Evaluate and summarize results in line with research goals.
  • Independently conduct, analyze, interpret in vitro and in vivo experiments. 
  • Summarize and present research results at internal and collaborative group meetings.

Basic Qualifications

A B.S. degree in the biological sciences and 3 years directly related research experience, including demonstrated skills in mammalian stem cell culture are required. 

Additional Qualifications and Skills

  • MS degree in biological sciences.
  • Experience maintaining mouse and human ES and iPS lines and in producing and maintaining differentiated cells, such as muscle satellite cells. 
  • Knowledge of high content screening, general familiarity with liquid handling robotics and reporter gene assays.
  • Strong organizational and oral, written, and graphical communication skills.
  • Ability to work with a multidisciplinary group of scientists in a dynamic team environment.
  • Ability to tackle multiple projects simultaneously.

Additional Information

This is a grant-funded two-year term position with the possibility of extension based on satisfactory performance and the continued availability of funding. 

The FAS and the Department of Stem Cell & Regenerative Biology are committed to cultivating not only the diversity of our faculty, staff, and students but also in developing an inclusive culture  that is vibrant, engaging and encouraging of innovation as well as intellectual debate. We believe creating and maintaining an inclusive workplace allows employees from all backgrounds and walks of life to achieve their fullest potential. We also believe an inclusive culture is one that accepts, values and views as strength the difference we all bring to the workplace. We encourage our community members to participate in our FAS Diversity Dialogues . 


USA - MA - Cambridge

Job Code

403125 Research Assistant IV (Lab)


Stem Cell and Regenerative Biology


55 - Hvd Union Cler & Tech Workers

Pre-Employment Screening

Education, Identity


Monday thru Friday 9am to 5pm

Some Holiday and Weekend Coverage may be Required 

EEO Statement

We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, gender identity, sexual orientation, pregnancy and pregnancy-related conditions, or any other characteristic protected by law.

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