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Stem Cell Research Associate I - 132069

UC San Diego Health

Job Description

UCSD Layoff from Career Appointment: Apply by 09/18/2024 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.

Special Selection Applicants: Apply by 09/27/2024. Eligible Special Selection clients should contact their Disability Counselor for assistance.

DESCRIPTION

UCSD Department of Psychiatry is one of the most innovative and productive academic departments in the country, offering challenging career opportunities in the diverse areas of advanced educational programs, cutting-edge research, and state-of-the-art clinical services. The department is committed to offering a dynamic learning environment and growing opportunities to its talented and dedicated employees.

Under supervision of the PI, the incumbent will work as a Stem Cell Research Associate 1 to perform routine molecular biology techniques such as aiding in maintaining human-derived iPSC colonies, as well as the generation of NPCs, brain organoids and brain assembloids within the Department of Psychiatry. The incumbent will assist PIs and other lab members with studies. Studies will include but are not limited to: 1) General growth, expansion, maintenance, of iPSCs; 2) Generation and characterization of NPCs, neurons, astrocytes, brain organoids and assembloids; 3) Characterization of resultant phenotypes (by molecular, multi-electrode array, and imaging techniques); and 4) Molecular biology techniques involving the isolation of DNA to identify genotype and visualization of phenotypes in the brain; 5) nuclei purification and preparation of libraries for single cell RNAseq and ATAcseq and proteomics. Procedures will include molecular biology (enzymatic extraction of DNA form tissue samples, PCR, gel electrophoresis), histology, light microscopy of sectioned organoids as well as basic molecular and cellular biology techniques (immunohistochemistry of organoid slices, Western blot, and maintenance of biochemistry equipment). Assist with project and other miscellaneous tasks as required by the supervisor and project scientists.

MINIMUM QUALIFICATIONS
  • Theoretical and/or practical knowledge in human pluripotent stem cells and neural differentiation.

  • Ability to perform molecular biology techniques including PCR, DNA preparation/extraction, gel electrophoresis.

  • Capable of performing biochemistry and laboratory techniques and demonstrated experience to prepare buffers and solutions, as well as various staining solutions.

  • Demonstrated experience with the operation of a personal computer and software application, such as Access, MS Word and Excel.

  • Proven ability to follow step based experimental protocols.

  • Ability to maintain effective working relationships with other staff members, students, faculty and scientists in a multi-cultural lab.

  • Experience with general lab maintenance including cleaning, stocking supplies.

  • Must be able to maintain confidentiality.

  • Ability to prioritize and perform efficiently.

  • Skill and ability to keep accurate data.

  • Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.

  • Ability to comply to UCSD policies and procedures and to stay informed and comply with basic safety rules and issues.

  • Working knowledge of standard molecular biology laboratory protocols (DNA/RNA extraction, PCR, immunohistochemistry, etc.); and culturing of human pluripotent stem cells (passing, freezing, differentiating).

  • Experience to assist with performing functional analyses and library preparation for single cell RNAseq, single cell ATACseq, proteomics, differentiation marker expression, multi-electrode arrays, CLARITY, immunohistochemistry of organoid slices.

SPECIAL CONDITIONS
  • Employment is subject to a criminal background check.

  • Willingness to work with standard hazardous laboratory compounds.

  • Willing to work evenings and weekends when necessary.

Pay Transparency Act

Annual Full Pay Range: $55,374 - $66,064 (will be prorated if the appointment percentage is less than 100%)

Hourly Equivalent: $26.52 - $31.64

Factors in determining the appropriate compensation for a role include experience, skills, knowledge, abilities, education, licensure and certifications, and other business and organizational needs. The Hiring Pay Scale referenced in the job posting is the budgeted salary or hourly range that the University reasonably expects to pay for this position. The Annual Full Pay Range may be broader than what the University anticipates to pay for this position, based on internal equity, budget, and collective bargaining agreements (when applicable).


UC San Diego Health is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, age, protected veteran status, gender identity or sexual orientation. For the complete University of California nondiscrimination and affirmative action policy see: http://www-hr.ucsd.edu/saa/nondiscr.html

 

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