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Research Assistant 2

Case Western Reserve University

Job Description

POSITION OBJECTIVE

Working under general supervision, the Research Assistant 2 will plan and carry out research project in accordance with general plans, related to multiple systems immunology tools, such as immunology, single cell RNA sequencing, proteomics, metabolomics, and metagenomics. The research projects are to understand host-microbe interactions that contribute to human reproductive health and disease. The research team collaborates with international partners in Africa, Europe, and Canada. The research assistant may work with mice. The research assistant may perform mouse basic husbandry, genotyping, research procedures including blood and tissue collection, anesthesia, viral or bacterial inoculations, injections, euthanasia, and necropsy. This position will work with animals.

 

ESSENTIAL FUNCTIONS

  • Plan and carry out project in accordance with general plans. Integrate multiple systems immunology tools (flow cytometry, proteomics, metabolomics, metagenomics) to study host-microbial interactions in human health. (15%)
  • Conduct a series of immunology, proteomic, and/or microbiome experiments to supply scientific information as part of a larger project. Sub-culture cell lines, analyze cell proliferation patterns, growth rates, etc.), conduct flow cytometry analysis, in vivo mouse models, conduct Western blots, DNA analysis, PCR, liquid chromatography, ELISA, mass spectrometry, and other compatible assays. (25%)
  • May assist in developing improved techniques, projection methods or procedures in DNA sequencing, flow cytometry, cell sorting, in vitro cell culture. (10%)
  • Collect and analyze samples for flow cytometry, protein/ metabolite analysis (mass spectrometry-based proteomics/ metabolomics), and/or sequencing (single cell RNA sequencing, 16S rRNA sequencing). Collect, analyze data, and create reports on activities to present at lab meetings. (15%)
  • Maintain equipment related to immunology, microbiome, or proteomics. Manage biohazardous waste and mouse colony. (10%)
  • Oversee inventory of supplies necessary for daily work, including but not limited to mass spectrometry sample preparation, tissue culture, flow cytometry, DNA analysis, and other related functions. (10%)
  • Train students, post-doctoral fellows and staff in techniques, data analysis, and utilization of equipment related to microbiome, proteomics, or immunology. Enforce occupational and environmental safety rules. (10%)
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    NONESSENTIAL FUNCITONS

    Perform other duties as assigned. (5%)

     

    CONTACTS

    Department: Department: Daily contact with senior research personnel and principal investigator regarding project activities. Frequent contact department administration to maintain workflow.

    University: Occasional contact with other researchers and collaborating departments regarding on-going project and lab activities.

    External: Occasional contact with vendors, IRB, other collaborating institutions regarding supplies and equipment needs.

    Students: Occasional contact with students to explain data, techniques, etc., and to maintain workflow.

     

    SUPERVISORY RESPONSIBILITIES

    This position has no direct supervision of staff employees. Train new staff; direct the work of students.

     

    QUALIFICAITONS

    Education and Experience: Bachelor of science degree and 1 to 3 years of experience or Associate's degree in an approved biotechnology program and 2 to 4 years of experience

     

    REQUIRED SKILLS

  • Has knowledge of commonly used concepts, practices, and procedures within immunology, microbiology, biochemistry, genetics, or related discipline.
  • Ability to rely on instructions and pre-established guidelines to perform the functions of the job.
  • Ability to operate laboratory equipment including flow cytometers and tissue culture systems.
  • Ability to ensure laboratory safety.
  • Ability to perform basic laboratory assays.
  • Must be highly motivated, responsible, dependable and a self-starter. Ability to meet consistent attendance.
  • Good/strong organization skills; ability to multi-task, prioritize and meet deadlines.  Must demonstrate attention to detail and accuracy, time management skills, and follow-through.  
  • Ability to work with sensitive information and maintain confidentiality. 
  • Excellent customer service skills; ability to maintain effective working relationships and address the potential and current needs of customers. Ability to work effectively independently and collaboratively within a team. Ability to interact with colleagues, supervisors, and customers face to face.
  • Effective problem-solving skills; must demonstrate sound judgment and good decision-making. 
  • Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
  • Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center’s mission. Must handle animals with care and respect at all times.
  • Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals.
  • Previous experience working with animals preferred.
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    WORKING CONDITIONS

    The employee will work mostly in Biosafety Level 2 (BSL-2) facilities and will use infectious materials, and general chemicals. This job may include the use of mouse models and will include handling of biological specimens that may be infected with HIV, HSV-2, malaria, TB, and other pathogens. May lift up to 24 lbs. This position may provide opportunities for international travel and presentations at international conferences.

    Case Western Reserve University’s animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the “Guide for the Care and Use of Laboratory Animals” appropriate Federal Animal Welfare Regulations, and the Public Health Service “Policy on the Humane Care and Use of Laboratory Animals.” This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.

     

    In employment, as in education, Case Western Reserve University is committed to Equal Opportunity and Diversity.  Women, veterans, members of underrepresented minority groups, and individuals with disabilities are encouraged to apply.

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    Case Western Reserve University provides reasonable accommodations to applicants with disabilities.  Applicants requiring a reasonable accommodation for any part of the application and hiring process should contact the Office of Equity at 216-368-3066 to request a reasonable accommodation.  Determinations as to granting reasonable accommodations for any applicant will be made on a case-by-case basis.

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