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Occupational Summary

The Asokan lab (www.asokanlab.org) is affiliated with the Duke Department of Surgery, Molecular Genetics & Microbiology & Biomedical Engineering and is focused on the development and evaluation of novel gene therapy vectors. We are currently seeking to hire a motivated candidate for a Research Technician II position to assist with molecular biology and in vivo murine studies in the lab. A competitive salary and benefits package will be provided according to the Duke University School of Medicine guidelines.

Work Performed

The candidate will work closely with lab members including postdoctoral fellows, graduate students, research technicians, and is expected to independently perform daily activities which include, but may not be limited to:

• Maintain mouse colony management including:
  • Record-keeping and inventory of the colony
  • Weaning, breeding, and monitoring mice
  • Collection and preparation of genotyping samples
• Perform a wide range of dosing and procedures in mouse models
• Obtain, prepare, mount and stain tissue, blood and other substances for microscopic examination.
• Assist with tissue harvests and processing; techniques include cryosectioning, immunohistochemistry, immunofluorescence, and other histology methods
• Experience with molecular biology techniques including: DNA/RNA extraction, PCR/qPCR, ELISA and Western blot
• Compile, document, and maintain precise records of experimental work
• Coordinate in vivo experiments with collaborators and industrial partners
• Drafting IACUC protocols, amendments, and renewals, and ensuring compliance with IACUC guidelines
• Operate laboratory instruments and equipment required for analysis, histology, microscopy, and Flow cytometry
• Assist in training new personnel.
• Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments.
• Attend and participate in relevant meetings, training, and development sessions.
• Perform other related duties to the work described herein

Education/Experience:

• Work requires a Bachelor’s degree in biology, genetics, zoology, microbiology or related scientific field.
• Must be detail-oriented, well organized
• Strong initiative and eagerness to learn
• Must adhere to strict lab safety requirements
• Ability to work and communicate effectively in a dynamic group environment.
• Previous experience with mouse handling and colony maintenance is required. Prior experience with molecular biology techniques, mouse injections and/or tissue processing is preferred.

Minimum Qualifications

Education

Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

Experience

None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Job Code: 00000951 RESEARCH TECHNICIAN II
Job Level: 08

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