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EPA Fellowship on Applying High Resolution Mass Spectrometry to Advance Inhalation Toxicity Screening: North Carolina
Job Description
- Agency
- U.S. Environmental Protection Agency (EPA)
- Location
- Research Triangle Park, North Carolina
- Job Category
- Internships
- Salary
- TBD
- Last Date to Apply
- 05/24/2023
- Website
- https://www.zintellect.com/Opportunity/Details/EPA-ORD-CPHEA-PHITD-2023-02
- Description
- *Applications may be reviewed on a rolling-basis and this posting could close before the deadline. Click here for information about the selection process. EPA Office/Lab and Location: A research opportunity is available at the Environmental Protection Agency (EPA), Office of Research and Development (ORD), Center for Public Health Environmental Assessment (CPHEA), Public Health and Integrated Toxicology Division (PHITD) located in Research Triangle Park, North Carolina. Research Project: Inhalation is the one of the three primary modes of chemical exposure; however, significant challenges in developing dosimetry methods, reproducible exposure systems, and appropriate in vitro models have led to its vast underrepresentation in EPA’s Tox21 initiatives. Methods to quantify deposition and/or cellular uptake following in vitro inhalation exposures at air-liquid interface (ALI) do not exist which prevents proper dose-response modeling. This is an important consideration given that studies typically use exposure concentration ([C]) as a surrogate for deposition which is often inaccurate. Given the remarkable improvements in Orbitrap high resolution mass spectrometry (HRMS), analytical quantification can now be achieved in complex matrices such as organotypic human airway models. We aim to develop analytical dosimetry methods that will quantify dose deposition, cellular uptake, and metabolism in human airway cells exposed to toxicants. This research project will combine ORD-developed ALI in vitro exposure technology, organotypic in vitro models of the human respiratory tract, and analytical dosimetry method development. The research participant will have the opportunity to collaborate with a multi-disciplinary team of toxicologists, biologists, chemists, and engineers and will utilize molecular, analytical, and computational approaches for in vitro assay development. Learning Objectives: With guidance from the mentor, the research participant will collaborate in developing methods, executing lab-based experiments, and generating, analyzing, and reporting data. Preferred areas of specialized training or experience include: mass spectrometry, cellular or tissue extraction methods for analytical methods, ALI cell culture, and cell-based assay development. Mentor(s): The mentor for this opportunity is Jessica Murray (murray.jessica@epa.gov). If you have questions about the nature of the research please contact the mentor(s). Anticipated Appointment Start Date: March 1, 2023. All start dates are flexible and vary depending on numerous factors. Click here for detailed information about start dates. Appointment Length: The appointment will initially be for one year and may be renewed upon EPA recommendation and subject to availability of funding. Level of Participation: The appointment is full-time. Participant Stipend: The participant will receive a monthly stipend commensurate with educational level and experience. Click here for detailed information about full-time stipends. EPA Security Clearance: Completion of a successful background investigation by the Office of Personnel Management (OPM) is required for an applicant to be on-boarded at EPA. ORISE Information: This program, administered by ORAU through its contract with the U.S. Department of Energy (DOE) to manage the Oak Ridge Institute for Science and Education (ORISE), was established through an interagency agreement between DOE and EPA. Participants do not become employees of EPA, DOE or the program administrator, and there are no employment-related benefits. Proof of health insurance is required for participation in this program. Health insurance can be obtained through ORISE. ORISE offers all ORISE EPA graduate students and Postdocs a free 5-year membership to the National Postdoctoral Association (NPA). The successful applicant(s) will be required to comply with Environmental, Safety and Health (ES&H) requirements of the hosting facility, including but not limited to, COVID-19 requirements (e.g. facial covering, physical distancing, testing, vaccination). Questions: Please see the FAQ section of our website. After reading, if you have additional questions about the application process please email ORISE.EPA.ORD@orau.org and include the reference code for this opportunity.
- Qualifications
- The qualified candidate should have received a bachelor's or master's degree in one of the relevant fields (e.g. Biochemistry, Chemistry), or be pursuing one of the degrees with completion before the appointment start date. Degree must have been received within five years of the appointment start date. Formal education and research experience in analytical chemistry with focus in mass spectrometry will enhance the research experience. Preferred skills/experience: - Experience with liquid chromatography mass spectrometry (LC-MS) - Experience in extraction and preparation of cell and tissue samples for LC-MS analysis - Experience with a Triple Quadrupole or Orbitrap Mass Spectrometry instrument - Experience with high resolution mass spectrometry (HRMS) for quantitative and qualitative analyses - Strong background in sample preparation and extraction procedures for LC-MS - Experience in instrument maintenance, solution preparation, and routine operation of analytical equipment - Method troubleshooting and validation - Data management, including written reports of laboratory activities, instrument status, system modifications, and data summaries - Skilled in quantitative analysis and Microsoft Excel
- Contact Person
- ORISE.EPA.ORD@orau.org
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