Research Technician - Pyle Lab


Job Description

Primary Work Address: Yale Medical School, New Haven, CT, 06536-1812

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The Pyle lab at Yale University explores RNA biology, studying the molecular interactions important for RNA structures and the activities of RNA-protein machines. Using tools that range from cryo-EM to cell culture, they seek to understand the impact of RNA architecture and dynamics on the life of the cell. In parallel, they develop new computational methods for solving and analyzing RNA structures. Their work has the potential to inform new strategies for the detection and treatment of viral and fungal pathogens such as hepatitis C virus, dengue virus, and Candida albicans . More information about the lab’s research can be found by visiting


About the Research Technician role:
Under the guidance of a senior lab member, this role will be responsible for performing a variety of techniques and tasks related to protein purification, tissue culture, cell line maintenance, and general lab operations support. This is an excellent opportunity for a hardworking, self-motivated professional looking for a long-term opportunity. Strong candidates will be organized, responsible, teamwork-oriented individuals who would enjoy working collaboratively with senior scientists, postdoctoral associates, other technicians, and students in support of research goals.

What we provide:

  • A competitive compensation package with comprehensive health and welfare benefits

  • Working with a diverse, collaborative team and lab environment

  • Hands-on training to learn a variety of molecular and cellular biology techniques, such as protein purification with fast protein liquid chromatography (FPLC) and bacterial and mammalian cell manipulation, as well as documentation skills

  • Opportunities for professional development and growth including the future potential to have your own research project and contribute to publications

What you’ll do:

  • Protein purification including maintaining a stock of basic proteins used in the lab

  • Tissue culture work including growing, maintaining, and harvesting common mammalian cells

  • Maintenance of AKTA instruments and columns, monitoring the user capabilities and checking the status of columns periodically

  • Preparation and maintenance of a stock of DH5alpha, BL21 DE3, EMBACY, Rosetta competent cells

  • Generation and maintenance of archives of cell lines, restriction enzymes, and antibodies

  • Preparation of common reagents and buffers

  • Making growth media and plates for lab members

  • General lab operations support including maintenance and sanitation of the E. coli room, monthly inventory checks and restocking, assisting others with ordering supplies, unpacking shipments, and glassware cleaning/autoclaving

What you bring:

  • A minimum of a bachelor's in the biological sciences. Specific coursework and/or training in biochemistry is a plus.

  • Previous experience with protein purification is strongly preferred.

  • Excellent verbal and written communication skills.

  • Excellent organizational skills, time management skills, and attention to detail. Ability to use good judgment in managing deadlines and responding to inquiries.

  • Proficient computer skills with demonstrated proficiency in Microsoft Office Suite.

The salary for this role in the state of Connecticut is $33,915 (minimum) - $42,400 (midpoint) - $55,125 (maximum)


HHMI’s salary structure is developed based on relevant market data. HHMI considers a candidates’ education, previous experiences, knowledge, skills and abilities, as well as internal equity when making job offers. Typically, a new Research Technician I is compensated between the minimum and the midpoint of the salary range.


Physical Requirements

Remaining in a normal seated or standing position for extended periods of time; reaching and grasping by extending hand(s) or arm(s); dexterity to manipulate objects with fingers, for example using a keyboard; communication skills using the spoken word; ability to see and hear within normal parameters; ability to move about workspace. The position requires mobility, including the ability to move materials weighing up to several pounds (such as a laptop computer or tablet). Work may involve possible exposure to malodorous vapors, low dose radiation, contamination by toxic chemicals and acids and presence of carcinogenic substances or other hazardous materials.

Persons with disabilities may be able to perform the essential duties of this position with reasonable accommodation. Requests for reasonable accommodation will be evaluated on an individual basis.

Please Note:

This job description sets forth the job’s principal duties, responsibilities, and requirements; it should not be construed as an exhaustive statement, however.  Unless they begin with the word “may,” the Essential Duties and Responsibilities described above are “essential functions” of the job, as defined by the Americans with Disabilities Act.

Compensation and Benefits

Our employees are compensated from a total rewards perspective in many ways for their contributions to our mission, including competitive pay, exceptional health benefits, retirement plans, time off, and a range of recognition and wellness programs. Visit our Benefits at HHMI site to learn more. 

HHMI is an Equal Opportunity Employer

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